If you submit new Medicago truncatula genes/mutants that are not published, please register them at the Medicago truncatula gene nomenclature Website first.

Please provide as much information as you can. Thanks.

1. Submitter Information

Name (First Last): (Required)

Email: (Required)

Department and Institute: (Required)

Telephone: (Required)

2. Mutant Information

Species: (Required) Medicago truncatula Sinorhizobium melilotiGlycine maxBradyrhizobium japonicum

Mutant Symbol: (Required, for plant, use mutant line, e.g. C71 (domi), for bacteria, user mutant strain, e.g. GMI5376)

Allele Symbol (for plant, e.g dmi1-1) or Genotype (for bacteria, e.g. delta(nodD1), nodH2212::Tn5): (Required)

Mutant Gene or Gene Group: (Required, e.g. dmi1, nodD1, nodD1D2D3)

Number of Genes Targeted: (Required, select one from the list)
12345678910

Mutagenesis: (Required, select one from the list)
EMS32P32P beta rays5-BromodeoxyuridineAntisenseChemicalcombination of multiple mutationsdeletiondominant negativeDouble cross-over deletionDouble cross-over insertionEI treatmentEMSFNBgamma ray, EMSgamma raysInsertionInterposonirradiationmicroRNAN-Methyl-N'-Nitro-N-NitrosoguanidineN-nitroso-N-methylureaNoneOtheroverexpressionradiationRNAisignature-tagged mutagenesisSilencingSingle cross-over insertionSite-directed MutagenesisSite-directed Mutagenesis using PCRsodium azide, ethyleneiminespontaneousT-DNATn5Tn5-233TransposonUncertainX rayX-ray, thermal neutron

Phenotype in Detail: (Required, e.g. Nod-; defective in calcium spiking; root hair tip swelling (Has) but are blocked for branching (Hab-); block in the induction of ENOD11 and ENOD40; dramatically reduced induction of RIP1; lack cortical cell division, do not form infection structures, no curling in response to live bacteria; Myc- phenotype; Recessive;)

Symbiotic Phenotype in Brief: (Required, e.g. Calcium flux+, Calcium spiking-, Has+, Hab-, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc-)

Mutant Class: (Required, select one from the list)
Nod- (No nodulation or nodulation is blocked at very a early stage)Nod+Fix- (Nodules do not fix nitrogen)Nod+Fix+- (Nodules with reduced activity of nitrogen fixation)Nod+Fix+ (Normal nodulation and nitrogen fixation)Nod+- (Smaller size or less number of nodules or delayed nodulation)Nod++ (Supernodulation or hypernodulation)

Additional information about the mutant: (Optional, e.g. hcl-2 is a leaky allele, etc.)

Allelism: (Optional, e.g. not allelic to dmi2, dmi3 or nsp1, determined by complementation test)

The earliest development stage known to be affected: (Optional, e.g. dmi1-1 is defect in Calcium spiking)
SELECT development_stage_id, development_stage FROM development_stage ORDER BY development_stage_id ASC ----------------------Host plant roots excrete FlavonoidExpression of bacterial nod genes and excretion of Nod factors Membrane depolarization and ion fluxesCalcium spikingRoot hair deformation and branchingENOD gene expressionCortical cell divisionsRoot hair curlingFormation of an infection threadNodule initiationBacteroid releaseBacteroid differentiationonset of nitrogen fixationComplementary functions associated with nitrogen fixationmaintenance (persistence) of nodule function

Target gene symbol 1 (Required, e.g. DMI1, nodD1):   

Target gene name 1 (Required, e.g. doesn't make infections, nodD1)   

Mutation location 1: (Optional, e.g. G1264A point mutation in dmi1.)

Target gene symbol 2:   

Target gene name 2:   

Mutation location 2:

Target gene symbol 3:   

Target gene name 3:   

Mutation location 3:

Target gene symbol 4:   

Target gene name 4:   

Mutation location 4:

Target gene symbol 5:   

Target gene name 5:   

Mutation location 5:

Wild type parent line (for plant) or strain (for bacteria): (Required, e.g. Rm1021, RCR2011, A17)

External URL link to this mutant: (Optional)

Reference List: Please list relevant paper and/or web references

Notes: