| Mutant Symbol | P1 |
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| Allele Symbol | dmi2-3 |
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| Gene and Mutation Location | DMI2 (NORK) (G to A transition in the splice consensus site of intron 5 caused the loss of exon 6 of 69 bp long from the mRNA) |
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| Symbiotic Phenotype Brief | Calcium flux+, Calcium spiking+-, Has+, Hab-, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc +/- ( leaky) |
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| Phenotype Detail | Nod-; leaky for calcium spiking; root hair tip swelling (Has) but are blocked for branching (Hab-); block in the induction of ENOD11 and ENOD40; dramatically reduced induction of RIP1; lack cortical cell division; no infection threads (Inf-); no root curling (Hac-) in response to live bacteria; Myc (+/-, leaky)phenotype; Recessive. A recent paper by Esseling et al., 2004 showed that when care is taken not to induce touch response, the mutant root hairs respond morphologically like the wild type to nod factor. |
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| Single gene targeted? | yes |
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| Extra Information | P1 is leaky mutant for calcium spiking, nodulation and mycorrhizal invasion. The calcium spiking experiments were performed on excised root segments (Wais et al, 2000). |
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| Mutagenesis Method | EMS |
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| Mutant Class | Nod- |
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| Allelism | not allelic to dmi1, dmi3 or nsp1, determined by complementation test |
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| The earliest development stage known to be affected | Calcium spiking |
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| Wildtype Line | Jemalong A17 |
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| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
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| Authors |
Year |
Title |
Locator |
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| Ane JM, Levy J, Thoquet P, Kulikova O, de Billy F, Penmetsa V, Kim DJ, Debelle F, Rosenberg C, Cook DR, Bisseling T, Huguet T and Denarie J | 2002 | Genetic and cytogenetic mapping of DMI1, DMI2, and DMI3 genes of Medicago truncatula involved in Nod factor transduction, nodulation, and mycorrhization | Mol Plant Microbe Interact. 15(11):1108-18 | | Catoira R, Galera C, de Billy F, Penmetsa RV, Journet EP, Maillet F, Rosenberg C, Cook D, Gough C and Denarie J | 2000 | Four genes of Medicago truncatula controlling components of a nod factor transduction pathway | Plant Cell. 12(9):1647-66 | | Endre G, Kereszt A, Kevei Z, Mihacea S, Kalo P and Kiss GB | 2002 | A receptor kinase gene regulating symbiotic nodule development | Nature. 417(6892):962-6 | | Esseling JJ, Lhuissier FG and Emons AM | 2004 | A nonsymbiotic root hair tip growth phenotype in NORK-mutated legumes: implications for nodulation factor-induced signaling and formation of a multifaceted root hair pocket for bacteria | Plant Cell. 16(4):933-44 | | Shaw SL and Long SR | 2003 | Nod factor elicits two separable calcium responses in Medicago truncatula root hair cells | Plant Physiol. 131(3):976-84 | | Wais RJ, Galera C, Oldroyd G, Catoira R, Penmetsa RV, Cook D, Gough C, Denarie J and Long SR | 2000 | Genetic analysis of calcium spiking responses in nodulation mutants of Medicago truncatula | Proc Natl Acad Sci U S A. 97(24):13407-12 |
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