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| Mutant Symbol | TR89 |
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| Allele Symbol | TR89 |
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| Gene and Mutation Location | DMI2 (NORK) |
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| Symbiotic Phenotype Brief | Nod+-Myc+- |
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| Phenotype Detail | TR89 belongs to mycorrhizal phenotype 3. Phenotype 3 showed these typical appressoria (thick lens-shaped with no intercellular hyphae developing from them) and normal appressoria leading to the formation of arbuscules on the same root system. For all the mutants belonging to mycorrhizal phenotype 3, the mycorrhizal fungus G. intraradices forms significantly much more typical AM structures than G. mosseae. They were scored as Nod- at 5 weeks, but developed a single or several nodules 10 weeks after sowing in greenhouse culture, in the presence of S. meliloti and absence of mineral nitrogen. For this reason, they were then classified as Nod+-. The few nodules were pinky, normally shaped and have nitrogen-fixing ability, as confirmed by the fact that plants became green again at the end of flowering. |
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| Single gene targeted? | yes |
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| Extra Information | TR89 was previously described as Myc+ (Sagan et al. 1998), Morandi et al. 2004 observed a Myc+- phenotype. This different result may be attributed to (1) the use of a very "efficient" inoculum in this new experiment, especially G. intraradices, which gives a very rapid and intense colonisation thus facilitating visualisation of arbuscules, (2) the fact that, in previous observations, the "search image" was different, since the concept of Myc+- had not been defined. |
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| Mutagenesis Method | gamma rays |
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| Mutant Class | Nod+- |
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| Allelism | |
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| The earliest development stage known to be affected | N/A |
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| Wildtype Line | Jemalong J5 |
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| Authors |
Year |
Title |
Locator |
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| Morandi D, Prado E, Sagan M, and Duc G | 2004 | Characterisation of new symbiotic Medicago truncatula (Gaertn.) mutants, and phenotypic or genotypic complementary information on previously described mutants. | Mycorrhiza | | Sagan M, de Larembergue H and Morandi D | 1998 | Genetic analysis of symbiosis mutants in Medicago truncatula | Elmerich C, Kondorosi A, Newton WE (eds) Biological nitrogen fixation for the 21st century. Kluwer, Dordrecht, pp 317?318 |
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| Data entered by | Jing Qiu (12/02/2004) |
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| Updated/Verified by | Jing Qiu (12/02/2004), Chunxia Wang (12/03/2004) |
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| Final verification status | verified by Chunxia Wang (12/03/2004) |
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