NodMutDB

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Mutant Detail



Mutant Detail:
Mutant Symbol4D-5 
Allele Symboldnf7 
Gene and Mutation LocationDNF7 
Symbiotic Phenotype BriefInf+Nod+Fix- 
Phenotype DetailThe mutant makes small, white nodules and has defects in nitrogen fixation. It can induce all the genes tested (MtLEC4, MtN31, MtCAM1, ENOD2, and MtLB1). It forms infection threads that proceed through the cortical cells and produce limited nodule-like structures. The infection threads are indistinguishable from those of wild-type plants. It is unknown whether bacteria can differentiate into bacteroids. Bacterial penetration into epidermal cells and into the outer cortical cells surrounding the nascent nodule of mutant plants is morphologically indistinguishable from those in wild-type plants. Bacterial colonization of nodule parenchyma cells is also indistinguishable between mutants and wild type. 
Single gene targeted?yes 
Extra InformationThe dnf7 mutant triggers reduced expression of nifH. 
Mutagenesis MethodFNB 
Mutant ClassNod+Fix- 
Allelismnot allelic to other DNFs 
The earliest development stage known to be affectedN/A 
Wildtype LineJemalong A17 
External Linkhttp://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html 

References:
Authors Year Title Locator
Mitra RM and Long SR 2004 Plant and Bacterial Symbiotic Mutants Define Three Transcriptionally Distinct Stages in the Development of the Medicago truncatula/Sinorhizobium meliloti Symbiosis Plant Physiol. 134(2):595-604 
Starker CG, Parra-Colmenares AL, Smith L, Mitra RM and Long SR 2006 Nitrogen Fixation Mutants of Medicago truncatula Fail to Support Plant and Bacterial Symbiotic Gene Expression Plant Physiol. 140(2):671-80 

Curation Information:
Data entered byJing Qiu (08/05/2004)
Updated/Verified byChunhong Mao (06/16/2006)
Final verification statusverified by Chunhong Mao (06/16/2006)