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| Mutant Symbol | ID-1 |
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| Allele Symbol | dnf1-1 |
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| Gene and Mutation Location | DNF1 |
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| Symbiotic Phenotype Brief | Inf+Nod+Fix- |
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| Phenotype Detail | The mutant makes small, white nodules and has defects in nitrogen fixation. It can induce MtLEC4 and ENOD2 but not MtN31, MtCAM1, or MtLB1. The mutant forms infection threads that proceed through the cortical cells and produce limited nodule-like structures. The infection threads are indistinguishable from those of wild-type plants. It is unknown whether bacteria can differentiate into bacteroids. The mutant creates larger nodule-like structures than those induced by early infection mutants. Bacterial penetration into epidermal cells and into the outer cortical cells surrounding the nascent nodule of mutant plants is morphologically indistinguishable from those in wild-type plants. Bacterial colonization of nodule parenchyma cells is also indistinguishable between mutants and wild type. |
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| Single gene targeted? | yes |
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| Extra Information | The dnf1 mutant is defective in the ability to elicit nifH expression. |
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| Mutagenesis Method | FNB |
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| Mutant Class | Nod+Fix- |
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| Allelism | not allelic to other DNFs |
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| The earliest development stage known to be affected | N/A |
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| Wildtype Line | Jemalong A17 |
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| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
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| Authors |
Year |
Title |
Locator |
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| Mitra RM and Long SR | 2004 | Plant and Bacterial Symbiotic Mutants Define Three Transcriptionally Distinct Stages in the Development of the Medicago truncatula/Sinorhizobium meliloti Symbiosis | Plant Physiol. 134(2):595-604 | | Starker CG, Parra-Colmenares AL, Smith L, Mitra RM and Long SR | 2006 | Nitrogen Fixation Mutants of Medicago truncatula Fail to Support Plant and Bacterial Symbiotic Gene Expression | Plant Physiol. 140(2):671-80 |
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| Data entered by | Jing Qiu (08/05/2004) |
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| Updated/Verified by | Jing Qiu (08/05/2004), Chunhong Mao (06/16/2006) |
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| Final verification status | verified by Chunhong Mao (06/16/2006) |
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