NodMutDB

NodMutDB

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since 4/20/2004

Last updated 10/01/09

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Mutant Detail

Mutant Detail:

Mutant Symbol	bit1-1
Allele Symbol	bit1-1
Gene and Mutation Location	ERN1 (BIT1, PDL) (the bit1-1 deletion affects at least five genes.)
Symbiotic Phenotype Brief	Inf-Nod-
Phenotype Detail	defective in infection; have defects in the ability of the infection thread to penetrate past the outer cortical cells of the plant; infection threads terminated prematurely with little expansion into the nodule primordium; inoculation with Sinorhizobium meliloti resulted in the formation of small bumps on the surface of the root. bit1-1 mutants never induced any of the nodulins tested (MtLEC4, MtN31, MtCAM1, ENOD2, or MtLB1). The mutants showed no or severely diminished ENOD11:GUS induction.
Single gene targeted?	yes
Extra Information
Mutagenesis Method	FNB
Mutant Class	Nod-
Allelism	not allelic to dmi1, dmi2, dmi3, nsp1,nsp2,hcl, rit or lin
The earliest development stage known to be affected	Formation of an infection thread
Wildtype Line	Jemalong A17
External Link	http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html

References:

Authors	Year	Title	Locator
Middleton PH, Jakab J, Penmetsa RV, Starker CG, Doll J, Kalo P, Prabhu R, Marsh JF, Mitra RM, Kereszt A, Dudas B, Vandenbosch K, Long SR, Cook DR, Kiss GB and Oldroyd GE	2007	An ERF Transcription Factor in Medicago truncatula That Is Essential for Nod Factor Signal Transduction	Plant Cell. 19(4):1221-34
Mitra RM and Long SR	2004	Plant and Bacterial Symbiotic Mutants Define Three Transcriptionally Distinct Stages in the Development of the Medicago truncatula/Sinorhizobium meliloti Symbiosis	Plant Physiol. 134(2):595-604

Curation Information:

Data entered by	Jing Qiu (08/05/2004)
Updated/Verified by	Chunhong Mao (08/22/2007)
Final verification status	verified by Chunhong Mao (08/22/2007)