The lin mutant shows root hair branching in response to inoculation of S. meliloti. The mutant develops visible nodule primordia, but has a reduced infection phenotype (Inf+-). The number of infection events is reduced relative to wild type, and those infections that do form are arrested soon after infection thread initiation. There are no infection threads in nodule primordia. Mycorrhizal infections of lin are normal.
Single gene targeted?
yes
Extra Information
The mutant showed wild-type basal levels and rhizobial induction of apyrase mRNA
Mutagenesis Method
EMS
Mutant Class
Nod-
Allelism
not allelic to dmi1 or pdl
The earliest development stage known to be affected
Navarro-Gochicoa MT, Camut S, Niebel A and Cullimore JV
2003
Expression of the apyrase-like APY1 genes in roots of Medicago truncatula is induced rapidly and transiently by stress and not by Sinorhizobium meliloti or Nod factors