| Mutant Symbol | bit1-2 (BC1F4) |
|---|
| Allele Symbol | bit1-2 (pdl1) |
|---|
| Gene and Mutation Location | ERN1 (BIT1, PDL) (located between the genetic markers TC87156_1 and TC80512_1 on LG7) |
|---|
| Symbiotic Phenotype Brief | Hab+Inf+-Ccd-Nod- |
|---|
| Phenotype Detail | root hair branching exists; develop numbers of infections comparable with wild-type plants, but fail to develop nodule primordia. The mutant displayed early arrest of infection thread growth, a phenotype reminiscent of bit1-1. width). In contrast with bit1-1, which had a wild-type root system in the absence of S. meliloti, the roots of bit1-2 had a much reduced root growth rate. Scoring for nodule structures and bacterial infection 21 d after inoculation revealed fully developed functional nodules in the wild type in contrast with bit1-2, which showed arrested infections and no noticeable inner cortical cell division. |
|---|
| Single gene targeted? | yes |
|---|
| Extra Information | bit1-2 is distinguished by abnormal root development. bit1-2 root hairs develop in alternating regions of dense and sparse hairs, giving the root a poodle's tail appearance hence, the previous reference to this allele as poodle (pdl). Cohn et al. (2001) showed no detectable MtAPY1 mRNA in the pdl mutant. In contrast, Navarro-Gochicoa et al. (2003) found that there was no great difference in the levels of MtAPY1 mRNA in roots compared with wild type. No explanation was given for the discrepancy between the two studies. |
|---|
| Mutagenesis Method | EMS |
|---|
| Mutant Class | Nod- |
|---|
| Allelism | not allelic to dmi1 or lin. |
|---|
| The earliest development stage known to be affected | Formation of an infection thread |
|---|
| Wildtype Line | Jemalong A17 |
|---|
| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
|---|
|
| Authors |
Year |
Title |
Locator |
|---|
| Cohn JR, Uhm T, Ramu S, Nam YW, Kim DJ, Penmetsa RV, Wood TC, Denny RL, Young ND, Cook DR, and Stacey G | 2001 | Differential regulation of a family of apyrase genes from Medicago truncatula | Plant Physiol. 125(4):2104-19 | | Middleton PH, Jakab J, Penmetsa RV, Starker CG, Doll J, Kalo P, Prabhu R, Marsh JF, Mitra RM, Kereszt A, Dudas B, Vandenbosch K, Long SR, Cook DR, Kiss GB and Oldroyd GE | 2007 | An ERF Transcription Factor in Medicago truncatula That Is Essential for Nod Factor Signal Transduction | Plant Cell. 19(4):1221-34 | | Navarro-Gochicoa MT, Camut S, Niebel A and Cullimore JV | 2003 | Expression of the apyrase-like APY1 genes in roots of Medicago truncatula is induced rapidly and transiently by stress and not by Sinorhizobium meliloti or Nod factors | Plant Physiol. 131(3):1124-36 |
|
