| Mutant Symbol | Y6 |
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| Allele Symbol | dmi1-3 |
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| Gene and Mutation Location | DMI1 (C913T point mutation that creates a premature stop codon truncating the protein at amino acid position 305) |
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| Symbiotic Phenotype Brief | Calcium flux+, Calcium spiking-, Has+, Hab-, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc- |
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| Phenotype Detail | Nod-; defective in calcium spiking; root hair tip swelling (Has) but are blocked for branching (Hab-); block in the induction of ENOD11 and ENOD40; dramatically reduced induction of RIP1; lack cortical cell division (Ccd-), no infection threads (Inf-), no root curling (Hac-) in response to live bacteria; Myc- phenotype; Recessive |
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| Single gene targeted? | yes |
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| Extra Information | A recent paper by Esseling et al., 2004 showed that DMI1 is not required for NF induced morphological responses. |
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| Mutagenesis Method | EMS |
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| Mutant Class | Nod- |
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| Allelism | not allelic to dmi2, dmi3 or nsp1, determined by complementation test |
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| The earliest development stage known to be affected | Calcium spiking |
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| Wildtype Line | Jemalong A17 |
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| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
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| Authors |
Year |
Title |
Locator |
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| Ane JM, Kiss GB, Riely BK, Penmetsa RV, Oldroyd GE, Ayax C, Levy J, Debelle F, Baek JM, Kalo P, Rosenberg C, Roe BA, Long SR, Denarie J and Cook DR | 2004 | Medicago truncatula DMI1 required for bacterial and fungal symbioses in legumes | Science. 303(5662):1364-7 | | Ane JM, Levy J, Thoquet P, Kulikova O, de Billy F, Penmetsa V, Kim DJ, Debelle F, Rosenberg C, Cook DR, Bisseling T, Huguet T and Denarie J | 2002 | Genetic and cytogenetic mapping of DMI1, DMI2, and DMI3 genes of Medicago truncatula involved in Nod factor transduction, nodulation, and mycorrhization | Mol Plant Microbe Interact. 15(11):1108-18 | | Catoira R, Galera C, de Billy F, Penmetsa RV, Journet EP, Maillet F, Rosenberg C, Cook D, Gough C and Denarie J | 2000 | Four genes of Medicago truncatula controlling components of a nod factor transduction pathway | Plant Cell. 12(9):1647-66 | | Esseling JJ, Lhuissier FG and Emons AM | 2004 | A nonsymbiotic root hair tip growth phenotype in NORK-mutated legumes: implications for nodulation factor-induced signaling and formation of a multifaceted root hair pocket for bacteria | Plant Cell. 16(4):933-44 | | Shaw SL and Long SR | 2003 | Nod factor elicits two separable calcium responses in Medicago truncatula root hair cells | Plant Physiol. 131(3):976-84 | | Wais RJ, Galera C, Oldroyd G, Catoira R, Penmetsa RV, Cook D, Gough C, Denarie J and Long SR | 2000 | Genetic analysis of calcium spiking responses in nodulation mutants of Medicago truncatula | Proc Natl Acad Sci U S A. 97(24):13407-12 |
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