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| Mutant Symbol | W1 |
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| Allele Symbol | hcl-2 |
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| Gene and Mutation Location | HCL |
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| Symbiotic Phenotype Brief | Calcium flux+, Calcium spiking+, Has+, Hab+, Enod gene expression+, Ccd+, Hac-, Inf-, Nod-, Myc+ |
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| Phenotype Detail | Nod-; calcium spiking, root hair branching, epidermal nodulin genes (MtENOD11, MtENOD12 and rip1) expression; cortical cell divisions (Ccd) are greater than B56 but lower than wild type and no meristem; defective in root hair curling; Myc+ |
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| Single gene targeted? | yes |
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| Extra Information | hcl-2 is a leaky allele |
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| Mutagenesis Method | EMS |
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| Mutant Class | Nod- |
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| Allelism | not allelic to dmi1, dmi2, dmi3, or nsp1, determined by complementation test |
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| The earliest development stage known to be affected | Root hair curling |
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| Wildtype Line | Jemalong A17 |
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| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
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| Authors |
Year |
Title |
Locator |
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| Catoira R, Timmers AC, Maillet F, Galera C, Penmetsa RV, Cook D, Denarie J and Gough C | 2001 | The HCL gene of Medicago truncatula controls Rhizobium-induced root hair curling | Development. 128(9):1507-18 | | Wais RJ, Galera C, Oldroyd G, Catoira R, Penmetsa RV, Cook D, Gough C, Denarie J and Long SR | 2000 | Genetic analysis of calcium spiking responses in nodulation mutants of Medicago truncatula | Proc Natl Acad Sci U S A. 97(24):13407-12 |
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| Data entered by | Chunhong Mao (05/06/2004) |
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| Updated/Verified by | Chunxia Wang (07/26/2004) |
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| Final verification status | verified by Chunxia Wang (07/26/2004) |
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