| Mutant Symbol | B56 |
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| Allele Symbol | hcl-1 |
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| Gene and Mutation Location | HCL |
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| Symbiotic Phenotype Brief | Calcium flux+, Calcium spiking+, Has+, Hab+, Enod gene expression+, Ccd+, Hac-, Inf-, Nod-, Myc+ |
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| Phenotype Detail | Nod-; calcium spiking, root hair branching, epidermal nodulin genes (MtENOD11, MtENOD12 and rip1) expression; cortical cell divisions (Ccd) are limited to the inner cortex; B56 has reduced Ccd foci in response to Nod factor; defective in root hair curling; Myc+ |
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| Single gene targeted? | yes |
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| Extra Information | B56 showed the most defective phenotype, both for root hair deformations and Ccd. hcl-1 is the most severe allele of the three hcl alleles (hcl-1, hcl-2, and hcl-3). The mutant exhibits a reduced transcriptional response to S. meliloti, which suggest that later stages of symbiotic development, involving root hair curling and cell divisions, require new gene expression. |
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| Mutagenesis Method | EMS |
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| Mutant Class | Nod- |
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| Allelism | not allelic to dmi1, dmi2, dmi3, or nsp1, determined by complementation test |
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| The earliest development stage known to be affected | Root hair curling |
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| Wildtype Line | Jemalong A17 |
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| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
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| Authors |
Year |
Title |
Locator |
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| Catoira R, Timmers AC, Maillet F, Galera C, Penmetsa RV, Cook D, Denarie J and Gough C | 2001 | The HCL gene of Medicago truncatula controls Rhizobium-induced root hair curling | Development. 128(9):1507-18 | | Mitra RM, Shaw SL and Long SR | 2004 | Six nonnodulating plant mutants defective for Nod factor-induced transcriptional changes associated with the legume-rhizobia symbiosis | Proc Natl Acad Sci U S A. 101(27):10217-22 | | Wais RJ, Galera C, Oldroyd G, Catoira R, Penmetsa RV, Cook D, Gough C, Denarie J and Long SR | 2000 | Genetic analysis of calcium spiking responses in nodulation mutants of Medicago truncatula | Proc Natl Acad Sci U S A. 97(24):13407-12 |
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