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| Mutant Symbol | 0-2 |
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| Allele Symbol | nsp2-1 |
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| Gene and Mutation Location | NSP2 (the sequence revealed a 435-bp deletion in the allele. The deletion removes a major portion of the conserved GRAS domain but does not induce a frameshift) |
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| Symbiotic Phenotype Brief | Calcium flux+, Calcium spiking+, Has+, Hab+, Enod gene expression-, Ccd-, Hac-, Inf-, Nod- |
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| Phenotype Detail | Nod-; calcium spiking equivalent to wild type; Altered root hair deformation (reduction in the level of root hair growth and deformation); Blocked for the formation of infection thread; Reduced early nodulin gene expression, complete block in ENOD11 expression and great reduced RIP1 expression; defective in cortical cell division and root hair curling |
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| Single gene targeted? | yes |
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| Extra Information | |
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| Mutagenesis Method | FNB |
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| Mutant Class | Nod- |
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| Allelism | not allelic to dmi1, dmi2, dmi3, nsp1 or hcl, determined by complementation test |
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| The earliest development stage known to be affected | ENOD gene expression |
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| Wildtype Line | Jemalong A17 |
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| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
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| Authors |
Year |
Title |
Locator |
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| Kalo P, Gleason C, Edwards A, Marsh J, Mitra RM, Hirsch S, Jakab J, Sims S, Long SR, Rogers J, Kiss GB, Downie JA and Oldroyd GE. | 2005 | Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators. | Science. 308(5729):1786-9. | | Oldroyd GE and Long SR | 2003 | Identification and characterization of nodulation-signaling pathway 2, a gene of Medicago truncatula involved in Nod actor signaling | Plant Physiol. 131(3):1027-32 |
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| Data entered by | Chunhong Mao (05/06/2004) |
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| Updated/Verified by | Chunxia Wang (07/26/2004), Jing Qiu (06/24/2005), Chunhong Mao (07/15/2005) |
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| Final verification status | verified by Chunhong Mao (07/15/2005) |
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