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since 4/20/2004

Last updated 10/01/09

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Mutant Detail

Mutant Detail:

Mutant Symbol	efd-1
Allele Symbol	efd-1
Gene and Mutation Location	EFD (The efd-1 deletion encompasses 1571 bp within the promoter (PEFD) and the coding region (EFD), including the ERF domain.)
Symbiotic Phenotype Brief	Nod++Fix-
Phenotype Detail	The efd-1 mutant produces more numerous nodules than wild type plants following S. meliloti inoculation (about threefold more) at 40 d after inoculation. This increased nodule density was accompanied by numerous infection threads particularly in the epidermis. Nodule primordia were more frequently infected by several infection threads in the efd-1 mutant than in the wild type, leading to broader or multilobed nodules with several meristems. efd-1 nodules were white and less elongated than wild-type nodules. They were shown to be defective in nitrogen fixation by an acetylene reduction assay. The bacteroid release structures (infection droplets) were often found to be much larger in efd-1 than in wild-type nodules and present not only in zone II but also in zone III, which was not observed in the wild-type control. The size of released bacteroids was normal, but the symbiosome membrane was often more difficult to see.
Single gene targeted?	yes
Extra Information
Mutagenesis Method	FNB
Mutant Class	Nod++
Allelism
The earliest development stage known to be affected	N/A
Wildtype Line	Jemalong A17

References:

Authors	Year	Title	Locator
Vernie T, Moreau S, de Billy F, Plet J, Combier JP, Rogers C, Oldroyd G, Frugier F, Niebel A and Gamas P	2008	EFD Is an ERF Transcription Factor Involved in the Control of Nodule Number and Differentiation in Medicago truncatula	Plant Cell. 20(10):2696-2713

Curation Information:

Data entered by	Chunhong Mao (01/08/2009)
Updated/Verified by	Chunhong Mao (01/08/2009)
Final verification status	to be verified