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| Mutant Symbol | CHS RNAi |
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| Allele Symbol | CHS RNAi |
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| Gene and Mutation Location | CHS (RNAi silencing) |
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| Symbiotic Phenotype Brief | Nod- |
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| Phenotype Detail | Silencing of CHS led to a near complete abolition of
nodulation of S. meliloti in M. truncatula hairy root suggesting that flavonoids are likely to be critical for nodulation in M. truncatula. The use of luteolin pre-treated S. meliloti cells significantly increased the number of nodules in CHSi hairy roots. However, the number of nodules on these roots was still significantly less than on vector control roots, suggesting that luteolin pre-treatment only partially restored nodulation in the absence of flavonoids in the root. This observation also suggests that the loss of nod gene inducers in the root exudates was not the sole reason for the loss of nodulation in CHSi roots, and that flavonoid compounds other than nod-gene-inducing flavones have an important and additive effect on nodule numbers. |
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| Single gene targeted? | yes |
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| Extra Information | CHS RNAi roots had about 40-fold less CHS transcripts than vector controls. The levels of all flavonoids in these
roots were significantly reduced. |
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| Mutagenesis Method | RNAi |
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| Mutant Class | Nod- |
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| Allelism | |
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| The earliest development stage known to be affected | N/A |
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| Wildtype Line | Jemalong A17 |
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| Authors |
Year |
Title |
Locator |
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| Zhang J, Subramanian S, Stacey G and Yu O | 2008 | Flavones and flavonols play distinct critical roles during nodulation of Medicago truncatula by Sinorhizobium meliloti | Plant J. |
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| Data entered by | Chunhong Mao (11/13/2008) |
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| Updated/Verified by | Chunhong Mao (11/13/2008) |
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| Final verification status | verified by Chunhong Mao (11/13/2008) |
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