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| Mutant Symbol | FNS RNAi |
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| Allele Symbol | FNS RNAi |
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| Gene and Mutation Location | FNS (RNAi silencing) |
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| Symbiotic Phenotype Brief | Nod+- |
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| Phenotype Detail | Silencing of FNS led to severely reduced nodulation using S. meliloti compared with vector control roots. The use of luteolin pre-treated S. meliloti cells did not restore the nodule numbers in FNSi roots compared with vector control roots suggesting that nod gene induction by flavones in the root exudates is important but not sufficient for the nodulation of M. truncatula. |
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| Single gene targeted? | yes |
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| Extra Information | FNS RNAi roots had about 20-fold less FNS transcripts than vector controls. The major difference in flavonoid profiles between CHRi and FNSi roots is that CHRi reduces the levels of both 5-deoxyisoflavonoids (e.g. formononetin) and Dhf, whereas FNSi only reduces the level of Dhf. |
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| Mutagenesis Method | RNAi |
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| Mutant Class | Nod+- |
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| Allelism | |
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| The earliest development stage known to be affected | N/A |
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| Wildtype Line | Jemalong A17 |
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| Authors |
Year |
Title |
Locator |
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| Zhang J, Subramanian S, Stacey G and Yu O | 2008 | Flavones and flavonols play distinct critical roles during nodulation of Medicago truncatula by Sinorhizobium meliloti | Plant J. |
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| Data entered by | Chunhong Mao (11/13/2008) |
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| Updated/Verified by | Chunhong Mao (11/13/2008) |
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| Final verification status | verified by Chunhong Mao (11/13/2008) |
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