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| Mutant Symbol | IFS RNAi |
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| Allele Symbol | IFS RNAi |
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| Gene and Mutation Location | IFS (RNAi silencing) |
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| Symbiotic Phenotype Brief | Nod+ |
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| Phenotype Detail | The silencing of IFS had no effect on nodulation using S. meliloti even though isoflavonoids were the major flavonoids in M. truncatula roots. IFS silencing had a more dramatic effect on the total flavonoid content in the roots than did FNS and CHR silencing. Yet, IFSi roots produced as many nodules as the vector control roots. This suggests that isoflavonoids are not likely to play a critical role during nodulation in M. truncatula. |
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| Single gene targeted? | yes |
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| Extra Information | IFS RNAi transgenic roots had a 15-fold reduction
in IFS transcript levels compared with vector control
transgenic roots, as measured by qRT-PCR. |
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| Mutagenesis Method | RNAi |
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| Mutant Class | Nod+Fix+ |
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| Allelism | |
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| The earliest development stage known to be affected | N/A |
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| Wildtype Line | Jemalong A17 |
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| Authors |
Year |
Title |
Locator |
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| Zhang J, Subramanian S, Stacey G and Yu O | 2008 | Flavones and flavonols play distinct critical roles during nodulation of Medicago truncatula by Sinorhizobium meliloti | Plant J. |
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| Data entered by | Chunhong Mao (11/13/2008) |
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| Updated/Verified by | Chunhong Mao (11/13/2008) |
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| Final verification status | verified by Chunhong Mao (11/13/2008) |
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