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Mutant Detail

Mutant Detail:

Mutant Symbol	35S:SINAT5DN
Allele Symbol	35S:SINAT5DN
Gene and Mutation Location	MtSINA1 (inhibited by a dominant negative form of the Arabidopsis SINAT5) MtSINA2 (inhibited by a dominant negative form of the Arabidopsis SINAT5) MtSINA3 (inhibited by a dominant negative form of the Arabidopsis SINAT5) MtSINA4 (inhibited by a dominant negative form of the Arabidopsis SINAT5) MtSINA5 (inhibited by a dominant negative form of the Arabidopsis SINAT5) MtSINA6 (inhibited by a dominant negative form of the Arabidopsis SINAT5)
Symbiotic Phenotype Brief	Nod+-Fix+-
Phenotype Detail	To acquire insight into the function of SINA proteins in nodulation, a dominant negative form of the Arabidopsis SINAT5 was ectopically expressed in the model legume M. truncatula. 10 plants of seven transgenic lines were grown under nitrogen-poor conditions and inoculated with S. meliloti 41 (pHC60-gfp; Cheng and Walker, 1998). An average of 4.22 +/- 0.29 nodules/plant was obtained for the 35S:SINAT5DN lines, whereas control plants had 8.53 +/- 1.02 nodules/plant at 22 dpi. Of the nodules that appeared on 35S:SINAT5DN plants, 40% were white, reflecting a lack of active leghemoglobin, a sign for Fix-. Fluorescence microscopy analysis of these nodules revealed that the central tissue was less fluorescent than that of the wild-type nodules, indicating that fewer bacteria had invaded the nodule. Besides the nodulation phenotype, transgenic plants were affected in shoot growth, leaf size, and lateral root number.
Single gene targeted?	no
Extra Information	M. truncatula R108 was selected for Agrobacterium tumefaciens-mediated transformation of the 35S:SINAT5DN construct.
Mutagenesis Method	dominant negative
Mutant Class	Nod+-
Allelism
The earliest development stage known to be affected	N/A
Wildtype Line	R108

References:

Authors	Year	Title	Locator
Den Herder G, De Keyser A, De Rycke R, Rombauts S, Van de Velde W, Clemente MR, Verplancke C, Mergaert P, Kondorosi E, Holsters M and Goormachtig S	2008	Seven in Absentia Proteins Affect Plant Growth and Nodulation in Medicago truncatula	Plant Physiol. 148(1):369-82

Curation Information:

Data entered by	Chunhong Mao (11/13/2008)
Updated/Verified by	Chunhong Mao (11/13/2008)
Final verification status	verified by Chunhong Mao (11/13/2008)