MtDVL1 (MtDVL1 is under the control of the 35S promoter)
Symbiotic Phenotype Brief
Nod+-
Phenotype Detail
No obvious phenotypic effect could be observed on root morphology or root growth when comparing overexpressing and empty vector control constructs. When transgenic roots were inoculated with S. meliloti, a clear reduction in the number of nodules formed was observed, with an average of 3.8-fold reduction for MtDVL1 overexpressing roots, compared with empty vector control. Nodule development and
nitrogen fixation on inoculated roots overexpressing MtDVL1
appeared normal when compared with control roots. A strong increase in the number of abortive infections was observed in roots overexpressing MtDVL1 (7-fold). In addition, the infection threads that generally aborted in the outer root cortex led to bulbous and sac-like arrested infection pockets.
Single gene targeted?
yes
Extra Information
Mutagenesis Method
overexpression
Mutant Class
Nod+-
Allelism
The earliest development stage known to be affected
N/A
Wildtype Line
Jemalong A17
References:
Authors
Year
Title
Locator
Combier JP, Kuster H, Journet EP, Hohnjec N, Gamas P and Niebel A
2008
Evidence for the involvement in nodulation of the two small putative regulatory peptide-encoding genes MtRALFL1 and MtDVL1
