NodMutDB

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Mutant Detail



Mutant Detail:
Mutant SymbolMtHAP2-1 RNAi-intron1 
Allele SymbolMtHAP2-1 RNAi-intron1 
Gene and Mutation LocationMtHAP2-1 (RNAi constructed using a sequence located in the first intron of MtHAP2-1) 
Symbiotic Phenotype BriefNod+- 
Phenotype DetailNodules formed on transgenic roots carrying the RNAi-intron1 silencing cassette are nitrogen fixing, with a normal differentiated structure and an active and persistent meristem. Nevertheless, due to the small size of these RNAi-intron1 nodules compared with control nodules, it is clear that growth has been significantly modified. Importantly, MtHAP2-1 transcript and protein levels in these nodules, 21 d after inoculation, were significantly higher than for wild-type. 
Single gene targeted?yes 
Extra Information 
Mutagenesis MethodRNAi 
Mutant ClassNod+- 
Allelism 
The earliest development stage known to be affectedN/A 
Wildtype LineJemalong A17 

References:
Authors Year Title Locator
Combier JP, de Billy F, Gamas P, Niebel A and Rivas S 2008 Trans-regulation of the expression of the transcription factor MtHAP2-1 by a uORF controls root nodule development Genes Dev. 22(11):1549-59 

Curation Information:
Data entered byChunhong Mao (07/01/2008)
Final verification statusto be verified