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| Mutant Symbol | B85 |
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| Allele Symbol | nsp1-1 |
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| Gene and Mutation Location | NSP1 (the mutant allele contains a premature stop codon and encodes a truncated protein of 239, instead of 554, amino acids) |
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| Symbiotic Phenotype Brief | Calcium flux+, Calcium spiking+, Has+, Hab+, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc+ |
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| Phenotype Detail | Nod-; calcium spiking equivalent to wild type; Display root hair branching; Reduced RIP1 and ENOD11 induction, no induction of ENDO40; defective in cortical cell division and root hair curling; Myc+; |
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| Single gene targeted? | yes |
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| Extra Information | The mutant showed no measurable transcriptional changes in response to S. meliloti inoculation. |
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| Mutagenesis Method | EMS |
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| Mutant Class | Nod- |
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| Allelism | not allelic to dmi1, dmi2, dmi3 or hcl, determined by complementation test |
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| The earliest development stage known to be affected | ENOD gene expression |
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| Wildtype Line | Jemalong A17 |
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| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
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| Authors |
Year |
Title |
Locator |
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| Catoira R, Galera C, de Billy F, Penmetsa RV, Journet EP, Maillet F, Rosenberg C, Cook D, Gough C and Denarie J | 2000 | Four genes of Medicago truncatula controlling components of a nod factor transduction pathway | Plant Cell. 12(9):1647-66 | | Mitra RM, Shaw SL and Long SR | 2004 | Six nonnodulating plant mutants defective for Nod factor-induced transcriptional changes associated with the legume-rhizobia symbiosis | Proc Natl Acad Sci U S A. 101(27):10217-22 | | Smit P, Raedts J, Portyanko V, Debelle F, Gough C, Bisseling T and Geurts R | 2005 | NSP1 of the GRAS protein family is essential for rhizobial Nod factor-induced transcription. | Science. 308(5729):1789-91. | | Wais RJ, Galera C, Oldroyd G, Catoira R, Penmetsa RV, Cook D, Gough C, Denarie J and Long SR | 2000 | Genetic analysis of calcium spiking responses in nodulation mutants of Medicago truncatula | Proc Natl Acad Sci U S A. 97(24):13407-12 |
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| Data entered by | Chunhong Mao (05/06/2004) |
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| Updated/Verified by | Chunxia Wang (07/26/2004), Jing Qiu (06/24/2005), Chunhong Mao (08/16/2005) |
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| Final verification status | verified by Chunhong Mao (08/16/2005) |
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