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since 4/20/2004

Last updated 10/01/09

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Mutant Detail

Mutant Detail:

Mutant Symbol	MtHMGR1 RNAi
Allele Symbol	MtHMGR1 RNAi
Gene and Mutation Location	MtHMGR1 (A 226-bp 59 untranslated region of MtHMGR1 was cloned in a pK7GWIWG2(II)-derived binary plasmid vector)
Symbiotic Phenotype Brief	Nod-
Phenotype Detail	In the roots of the Jemalong A17 plants transformed with pRNAiMtHMGR1, the expression of MtHMGR1 was reduced on average to 40% of the wild type level. No nodules appeared on the cotransformed transgenic roots expressing pRNAiMtHMGR1, while on the same plant, roots that were not cotransformed with pRNAiMtHMGR1 developed a comparable number of nodules as the controls.
Single gene targeted?	yes
Extra Information	A 226-bp 59 untranslated region of MtHMGR1 was cloned in a pK7GWIWG2(II)-derived binary plasmid vector carrying the DsRED1 fluorescent marker resulting in the pRNAiMtHMGR1 construct. Wild-type (Jemalong A17) plants were transformed with this construct.
Mutagenesis Method	RNAi
Mutant Class	Nod-
Allelism
The earliest development stage known to be affected	N/A
Wildtype Line	Jemalong A17

References:

Authors	Year	Title	Locator
Kevei Z, Lougnon G, Mergaert P, Horvath GV, Kereszt A, Jayaraman D, Zaman N, Marcel F, Regulski K, Kiss GB, Kondorosi A, Endre G, Kondorosi E and Ane JM	2007	3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase1 Interacts with NORK and Is Crucial for Nodulation in Medicago truncatula	Plant Cell.

Curation Information:

Data entered by	Chunhong Mao (01/23/2008)
Final verification status	to be verified