NodMutDB

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since 4/20/2004

Last updated 10/01/09

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Mutant Detail

Mutant Detail:

Mutant Symbol	TRV25 pRNAiIPD3
Allele Symbol	dmi3-1 ipd3-RNAi
Gene and Mutation Location	DMI3 (A 14-bp deletion (TGAAGAGTTTACTG) in the gene caused translational termination after approximately two-third of the kinase domain, presumably resulting in a nonfunctional or a calcium/CaM-independent kinase) IPD3 (IPD3 knockdown by RNAi)
Symbiotic Phenotype Brief	Nod-
Phenotype Detail	Nodules were never observed on control nontransgenic roots of dmi3 plants nor on dmi3 plants transformed with pRNAiIPD3.
Single gene targeted?	no
Extra Information	dmi3 plants were transformed with pRNAiIPD3 to knockdown IPD3.
Mutagenesis Method	RNAi
Mutant Class	Nod-
Allelism
The earliest development stage known to be affected	N/A
Wildtype Line	Jemalong A17

References:

Authors	Year	Title	Locator
Messinese E, Mun JH, Yeun LH, Jayaraman D, Rouge P, Barre A, Lougnon G, Schornack S, Bono JJ, Cook DR and Ane JM	2007	A novel nuclear protein interacts with the symbiotic DMI3 calcium- and calmodulin-dependent protein kinase of Medicago truncatula	Mol Plant Microbe Interact. 20(8):912-21

Curation Information:

Data entered by	Chunhong Mao (08/30/2007)
Updated/Verified by	Chunhong Mao (08/30/2007)
Final verification status	to be verified