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Last updated 10/01/09

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Mutant Detail

Mutant Detail:

Mutant Symbol	12S
Allele Symbol	Mtnin-1
Gene and Mutation Location	MtNIN (an 11-bp deletion starting at position 1850 at MtNIN locus)
Symbiotic Phenotype Brief	Ccd- Inf- Nod-
Phenotype Detail	12S is Nod-. S. meliloti did not stimulate cortical cell divisions in these mutants, even at a time point in which wild-type plants had fully formed nodules. The mutant also exhibited the characteristic nin mutant phenotype of excessive root hair curling in response to S. meliloti. The formation of infection foci nor threads was observed in the mutant 12S. The mutant formed normal mycorrhizas. Mtnin-1 exhibited a normal calcium oscillatory response following NF treatment. MtNIN is not required for NF-induced gene expression. ENOD11 spatial expression is dramatically altered in Mtnin-1. Out of more than 85 independently transformed Mtnin-1 composite plants, none formed spontaneous nodules.
Single gene targeted?	yes
Extra Information	The F1 progeny of reciprocal crosses between Mtnin-1 and Mtnin-2 were Nod- demonstrating allelism. Mtnin-1 expresses the stronger phenotype of the two alleles.
Mutagenesis Method	FNB
Mutant Class	Nod-
Allelism	not allelic to dmi1, dmi2, dmi3, nfp, nsp1, nsp2, hcl. Mtnin-1 and Mtnin-2 are allelic.
The earliest development stage known to be affected	Cortical cell divisions
Wildtype Line	Jemalong A17

References:

Authors	Year	Title	Locator
Marsh JF, Rakocevic A, Mitra RM, Brocard L, Sun J, Eschstruth A, Long SR, Schultze M, Ratet P and Oldroyd GE	2007	Medicago truncatula NIN Is Essential for Rhizobial-Independent Nodule Organogenesis Induced by Autoactive Calcium/Calmodulin-Dependent Protein Kinase	Plant Physiol. 144(1):324-35

Curation Information:

Data entered by	Chunhong Mao (08/23/2007)
Final verification status	to be verified