ENOD40-1 (A. rhizogenes-mediated RNAi using pRRsil4012) ENOD40-2 (A. rhizogenes-mediated RNAi using pRRsil4012)
Symbiotic Phenotype Brief
Inf+Bad+-Nod+-
Phenotype Detail
In roots containing pRRsil4012 DNA, the level of the transcript of MtENOD40-1 and MtENOD40-2 is reduced >5-fold compared with the transcript levels of MtENOD40-1 and MtENOD40-2 in control roots. On roots, in which the expression of both MtENOD40-1 and MtENOD40-2 is reduced, the average number of nodules per root is 1.5 (75% reduction). Thus knocking down of the expression of both genes has an additive effect, suggesting that MtENOD40 acts in a dose-dependent manner. Analyses of nodules in which the expression of both MtENOD40 genes is reduced also showed that bacteroid development was blocked at stage II-III.
Single gene targeted?
no
Extra Information
Vector pRRsil4012 was used to knock down both MtENOD40 genes.
Mutagenesis Method
RNAi
Mutant Class
Nod+-
Allelism
The earliest development stage known to be affected
Bacteroid differentiation
Wildtype Line
Jemalong A17
References:
Authors
Year
Title
Locator
Wan X, Hontelez J, Lillo A, Guarnerio C, van de Peut D, Fedorova E, Bisseling T and Franssen H
2007
Medicago truncatula ENOD40-1 and ENOD40-2 are both involved in nodule initiation and bacteroid development
