ENOD40-2 (A. rhizogenes-mediated RNAi using pRRsil402)
Symbiotic Phenotype Brief
Inf+Bad+-Nod+-
Phenotype Detail
The transcript level of MtENOD40-2 was reduced about 5- to 25-fold in RNA isolated from MtENOD40-2 RNAi roots as compared with control roots. Knock-down of MtENOD40-2 expression led to a 38.5% reduction in the number of nodules. The nodules formed on MtENOD40-2 RNAi plants are spherical and small. Analyses of MtENOD40-2 RNAi and nodules in which the expression of both MtENOD40 genes is reduced also showed that bacteroid development was blocked at stage II-III. These data indicate that reduction of the MtENOD40-2 expression levels in nodules interfered with bacteroid development.
Single gene targeted?
yes
Extra Information
A. rhizogenes-mediated RNAi was applied in M. truncatula hairy roots. Vector (pRRsil402) was used to reduce expression of MtENOD40-2.
Mutagenesis Method
RNAi
Mutant Class
Nod+-
Allelism
The earliest development stage known to be affected
Bacteroid differentiation
Wildtype Line
Jemalong A17
References:
Authors
Year
Title
Locator
Wan X, Hontelez J, Lillo A, Guarnerio C, van de Peut D, Fedorova E, Bisseling T and Franssen H
2007
Medicago truncatula ENOD40-1 and ENOD40-2 are both involved in nodule initiation and bacteroid development
