NodMutDB

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since 4/20/2004

Last updated 10/01/09

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Mutant Detail

Mutant Detail:

Mutant Symbol	MtHAP2-1 RNAi
Allele Symbol	MtHAP2-1 RNAi
Gene and Mutation Location	MtHAP2-1 (RNA interference of MtHAP2-1 gene)
Symbiotic Phenotype Brief	Bar-Bad-Nod+-Fix-
Phenotype Detail	A reduction of MtHAP2-1 expression by RNAi was sufficient to significantly alter nodule development. Nodulation was delayed and nodule growth arrested at ∼8?10 d post-inoculation. Inside the resulting spherical nodular structures, the absence of the clearly delimited zones found in wildtype nodules indicated an abnormal nodule developmental process. Growth arrest was often associated with the development of a closed endodermal layer surrounding the nodules. In addition, cells of the apical meristematic zone lost their typical polyhedral shape and accumulated numerous enlarged vacuoles. MtHAP2-1 RNAi nodules were also unable to fix nitrogen as shown by the reduced development of plants grown in the absence of mineral nitrogen. This phenotype was confirmed by electron microscopy observations showing that in the infection zone of mature MtHAP2-1-RNAi nodules, bacteria were not released from infection threads.
Single gene targeted?	yes
Extra Information
Mutagenesis Method	RNAi
Mutant Class	Nod+Fix-
Allelism
The earliest development stage known to be affected	Bacteroid release
Wildtype Line	Jemalong A17

References:

Authors	Year	Title	Locator
Combier JP, Frugier F, de Billy F, Boualem A, El-Yahyaoui F, Moreau S, Vernie T, Ott T, Gamas P, Crespi M and Niebel A	2006	MtHAP2-1 is a key transcriptional regulator of symbiotic nodule development regulated by microRNA169 in Medicago truncatula	Genes Dev. 20(22):3084-8

Curation Information:

Data entered by	Chunhong Mao (12/15/2006)
Updated/Verified by	Chunhong Mao (12/15/2006)
Final verification status	verified by Chunhong Mao (12/15/2006)