| Mutant Symbol | MtCRE1 RNAi |
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| Allele Symbol | MtCRE1 RNAi |
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| Gene and Mutation Location | MtCRE1 (RNA interference) |
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| Symbiotic Phenotype Brief | Nod+- |
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| Phenotype Detail | MtCRE1 RNA interference (RNAi) roots showed strong reduction in their cytokinin sensitivity and in their primary response to this hormone. These roots also showed a significant increase in lateral root development and a reduction in nodulation. In the in vitro growth conditions, 50% of the Mt CRE1 RNAi roots did not develop any nodule. Both S. meliloti infection and early nodule organogenesis are perturbed in MtCRE1 RNAi roots. |
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| Single gene targeted? | yes |
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| Extra Information | Cytokinin signaling mediated by a single receptor, MtCRE1, leads to an opposite control of symbiotic nodule and lateral root organogenesis. Mt NIN, Mt RR1, and Mt RR4 define a common pathway activated during early S. meliloti interaction, allowing crosstalk between plant cytokinins and bacterial Nod factors signals. |
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| Mutagenesis Method | RNAi |
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| Mutant Class | Nod+- |
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| Allelism | |
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| The earliest development stage known to be affected | N/A |
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| Wildtype Line | Jemalong A17 |
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