Roots transformed with DMI3 delta(328-355) showed strong ENOD11-GUS expression in epidermal cells, primarily of lateral roots, in the absence of Nod factor treatment 36% of root systems. The ENOD11-GUS expression induced by the deletion construct was observed in a similar zone of epidermal cells to that of the ENOD11 induction by Nod factor. No bacterially infected nodules were found in complementation tests with dmi3-1.
Single gene targeted?
yes
Extra Information
The CaM binding/autoinhibition domain of the DMI3 protein is deleted. The deletion leads to constitutive substrate phosphorylation in vitro that is independent of Ca2+ and CaM. The DMI3 delta(328-355) construct is driven by the 35S promoter.
Mutagenesis Method
deletion
Mutant Class
Nod+Fix-
Allelism
The earliest development stage known to be affected
N/A
Wildtype Line
Jemalong J5
References:
Authors
Year
Title
Locator
Gleason C, Chaudhuri S, Yang T, Munoz A, Poovaiah BW and Oldroyd GE
2006
Nodulation independent of rhizobia induced by a calcium-activated kinase lacking autoinhibition
