NodMutDB

Query NodMutDB

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Mutant Detail



Mutant Detail:
Mutant SymbolR38 
Allele Symboldmi2-4 
Gene and Mutation LocationDMI2 (NORK) (Conserved Gly changed to Glu in the kinase subdomain X which might interact with the substrate) 
Symbiotic Phenotype BriefCalcium flux+, Calcium spiking-, Has+, Hab-, Enod gene induction-, Ccd-, Hac-, Inf-, Nod-, Myc+ 
Phenotype DetailNod-; defective in calcium spiking; root hair tip swelling (Has) but are blocked for branching (Hab-); block in the induction of ENOD11 and ENOD40; dramatically reduced induction of RIP1; lack cortical cell division; no infection threads (Inf-); no root curling (Hac-) in response to live bacteria; Myc+ phenotype; Recessive 
Single gene targeted?yes 
Extra InformationA recent paper by Esseling et al., 2004 showed that when care is taken not to induce touch response, the dmi2 mutant root hairs respond morphologically like the wild type to nod factor. 
Mutagenesis MethodEMS 
Mutant ClassNod- 
Allelismnot allelic to dmi1, dmi3 or nsp1, determined by complementation test 
The earliest development stage known to be affectedCalcium spiking 
Wildtype LineJemalong A17 
External Linkhttp://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html 

References:
Authors Year Title Locator
Endre G, Kereszt A, Kevei Z, Mihacea S, Kalo P and Kiss GB 2002 A receptor kinase gene regulating symbiotic nodule development Nature. 417(6892):962-6 
Esseling JJ, Lhuissier FG and Emons AM 2004 A nonsymbiotic root hair tip growth phenotype in NORK-mutated legumes: implications for nodulation factor-induced signaling and formation of a multifaceted root hair pocket for bacteria Plant Cell. 16(4):933-44 

Curation Information:
Data entered byChunhong Mao (05/06/2004)
Updated/Verified byChunxia Wang (07/26/2004), Chunhong Mao (09/02/2004)
Final verification statusverified by Chunhong Mao (09/02/2004)