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| Mutant Symbol | C31 |
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| Allele Symbol | nfp-1 |
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| Gene and Mutation Location | NFP (a mutation in residue no. 1 gives rise to a stop codon) |
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| Symbiotic Phenotype Brief | Calcium flux-, Calcium spiking-, Has-, Hab-, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc+ |
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| Phenotype Detail | Nod-; defective in NF induced rapid calcium flux, calcium spiking, root hair deformation, NF induced nodulin gene expression (rip1, MtENOD11 and MtENOD40), cortical cell divisions, root hair curling and infection thread initiation. The nfp mutant shows a wild type mycorrhizal phenotype, and MtENOD11 is expressed during the mycorrhizal colonisation. |
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| Single gene targeted? | yes |
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| Extra Information | C31 is the first Nod- mutant identified that is blocked for the induction of a rapid calcium flux in response to Nod factors.
NFP codes for a LysM receptor-like kinase that did not show autophosphorylation activity and that is expressed throughout the nodulation process. |
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| Mutagenesis Method | EMS |
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| Mutant Class | Nod- |
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| Allelism | not allelic to dmi1, dmi2, dmi3, nsp1, nsp2 or hcl determined by complementation test |
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| The earliest development stage known to be affected | Membrane depolarization and ion fluxes |
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| Wildtype Line | Jemalong A17 |
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| External Link | http://www.genome.clemson.edu/affiliated_cugi/medicago/genelist.html |
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| Authors |
Year |
Title |
Locator |
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| Amor BB, Shaw SL, Oldroyd GE, Maillet F, Penmetsa RV, Cook D, Long SR, Denarie J and Gough C | 2003 | The NFP locus of Medicago truncatula controls an early step of Nod factor signal transduction upstream of a rapid calcium flux and root hair deformation | Plant J. 34(4):495-506 | | Arrighi JF, Barre A, Ben Amor B, Bersoult A, Soriano LC, Mirabella R, de Carvalho-Niebel F, Journet EP, Gherardi M, Huguet T, Geurts R, Denarie J, Rouge P and Gough C | 2006 | The Medicago truncatula LysM-receptor Kinase Gene Family Includes NFP and New Nodule-expressed Genes | Plant Physiol. |
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| Data entered by | Chunhong Mao (05/06/2004) |
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| Updated/Verified by | Chunxia Wang (07/26/2004), Jing Qiu (10/13/2004), Chunhong Mao (08/07/2006) |
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| Final verification status | verified by Chunhong Mao (08/07/2006) |
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