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Last updated 10/01/09

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Mutant List

Mutants for EFD: 3

Mutant Symbol	Allele or Genotype	Mutant Gene	Phenotype Detail	Symbiotic Phenotype Brief	Mutagenesis Method	Mutant Class	WT Strain or Line
EFD RNAi	EFD RNAi	efd	M. truncatula roots were transformed with an EFD RNA interference (RNAi) construct expressed under the control of the 35S cauliflower mosaic virus promoter. A significant increase in nodulation and in the number of infection threads was observed in these roots, corresponding to a weaker efd mutant allele.	Nod++	RNAi	Nod++	Jemalong A17
EFD:VP16	EFD:VP16	efd	In a nodulation time course, there was a statistically significant threefold reduction in the number of nodules with the EFD:VP16 construct compared with roots expressing the VP16 domain alone, accompanied by a 3.5-fold reduction in the number of infection threads in roots. A detailed examination of infections showed that the ratio of epidermal versus cortical infection threads was similar in wild-type and EFD-overexpressing roots.	Nod+-	overexpression	Nod+-	Jemalong A17
efd-1	efd-1	efd	The efd-1 mutant produces more numerous nodules than wild type plants following S. meliloti inoculation (about threefold more) at 40 d after inoculation. This increased nodule density was accompanied by numerous infection threads particularly in the epidermis. Nodule primordia were more frequently infected by several infection threads in the efd-1 mutant than in the wild type, leading to broader or multilobed nodules with several meristems. efd-1 nodules were white and less elongated than wild-type nodules. They were shown to be defective in nitrogen fixation by an acetylene reduction assay. The bacteroid release structures (infection droplets) were often found to be much larger in efd-1 than in wild-type nodules and present not only in zone II but also in zone III, which was not observed in the wild-type control. The size of released bacteroids was normal, but the symbiosome membrane was often more difficult to see.	Nod++Fix-	FNB	Nod++	Jemalong A17

References:

Authors	Year	Title	Locator
Vernie T, Moreau S, de Billy F, Plet J, Combier JP, Rogers C, Oldroyd G, Frugier F, Niebel A and Gamas P	2008	EFD Is an ERF Transcription Factor Involved in the Control of Nodule Number and Differentiation in Medicago truncatula	Plant Cell. 20(10):2696-2713