| Mutant Symbol |
Allele or Genotype |
Mutant Gene |
Phenotype Detail |
Symbiotic Phenotype Brief |
Mutagenesis Method |
Mutant Class |
WT Strain or Line |
|---|
| CHS RNAi | CHS RNAi | chs | Silencing of CHS led to a near complete abolition of
nodulation of S. meliloti in M. truncatula hairy root suggesting that flavonoids are likely to be critical for nodulation in M. truncatula. The use of luteolin pre-treated S. meliloti cells significantly increased the number of nodules in CHSi hairy roots. However, the number of nodules on these roots was still significantly less than on vector control roots, suggesting that luteolin pre-treatment only partially restored nodulation in the absence of flavonoids in the root. This observation also suggests that the loss of nod gene inducers in the root exudates was not the sole reason for the loss of nodulation in CHSi roots, and that flavonoid compounds other than nod-gene-inducing flavones have an important and additive effect on nodule numbers. | Nod- | RNAi | Nod- | Jemalong A17 |
|
