With the exception of the reduced length of root hairs, no
notable differences in shoot or root growth and overall development were observed for api mutant BC2 plants prior to
inoculation. The api mutant phenotype, characterized by small and noninvaded nodular outgrowths, was clearly visible as early as 3 days postinoculation. The rhizobial infection was also defective at the earlier stage of infection thread (IT) initiation in root hairs, as well as later during IT growth in the small percentage of nodules which overcome the primordium invasion block. Nodulated api plants are not self-sufficient for nitrogen fixation. Neither modulating ethylene biosynthesis nor reducing ethylene sensitivity in a skl genetic background alters the basic api phenotype, suggesting that API function is not closely linked to ethylene metabolism or signaling.
T1-3 had numerous small white outgrowths along the root
system following inoculation with Sinorhizobium meliloti. Although the majority (>85%) of these outgrowths were not invaded by rhizobia, arrested infections were always observed in the outer cortex. T1-3 also showed reduced lateral root elongation and shorter root hairs compared with the wild type.
Nod+-
EMS
Nod+-
Jemalong A17
References:
Authors
Year
Title
Locator
Teillet A, Garcia J, de Billy F, Gherardi M, Huguet T, Barker DG, de Carvalho-Niebel F and Journet EP
2008
api, A Novel Medicago truncatula Symbiotic Mutant Impaired in Nodule Primordium Invasion
