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Last updated 10/01/09

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Mutant List

Mutants for ENOD40-2: 2

Mutant Symbol	Allele or Genotype	Mutant Gene	Phenotype Detail	Symbiotic Phenotype Brief	Mutagenesis Method	Mutant Class	WT Strain or Line
MtENOD40-1 MtENOD40-2 RNAi	MtENOD40-1 MtENOD40-2 RNAi	ENOD40-1ENOD40-2	In roots containing pRRsil4012 DNA, the level of the transcript of MtENOD40-1 and MtENOD40-2 is reduced >5-fold compared with the transcript levels of MtENOD40-1 and MtENOD40-2 in control roots. On roots, in which the expression of both MtENOD40-1 and MtENOD40-2 is reduced, the average number of nodules per root is 1.5 (75% reduction). Thus knocking down of the expression of both genes has an additive effect, suggesting that MtENOD40 acts in a dose-dependent manner. Analyses of nodules in which the expression of both MtENOD40 genes is reduced also showed that bacteroid development was blocked at stage II-III.	Inf+Bad+-Nod+-	RNAi	Nod+-	Jemalong A17
MtENOD40-2 RNAi	MtENOD40-2 RNAi	ENOD40-2	The transcript level of MtENOD40-2 was reduced about 5- to 25-fold in RNA isolated from MtENOD40-2 RNAi roots as compared with control roots. Knock-down of MtENOD40-2 expression led to a 38.5% reduction in the number of nodules. The nodules formed on MtENOD40-2 RNAi plants are spherical and small. Analyses of MtENOD40-2 RNAi and nodules in which the expression of both MtENOD40 genes is reduced also showed that bacteroid development was blocked at stage II-III. These data indicate that reduction of the MtENOD40-2 expression levels in nodules interfered with bacteroid development.	Inf+Bad+-Nod+-	RNAi	Nod+-	Jemalong A17

References:

Authors	Year	Title	Locator
Wan X, Hontelez J, Lillo A, Guarnerio C, van de Peut D, Fedorova E, Bisseling T and Franssen H	2007	Medicago truncatula ENOD40-1 and ENOD40-2 are both involved in nodule initiation and bacteroid development	J Exp Bot.