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Mutant List



Mutants for DNF1: 2
Mutant Symbol Allele or Genotype Mutant Gene Phenotype Detail Symbiotic Phenotype Brief Mutagenesis Method Mutant Class WT Strain or Line
ID-1 dnf1-1 dnf1 The mutant makes small, white nodules and has defects in nitrogen fixation. It can induce MtLEC4 and ENOD2 but not MtN31, MtCAM1, or MtLB1. The mutant forms infection threads that proceed through the cortical cells and produce limited nodule-like structures. The infection threads are indistinguishable from those of wild-type plants. It is unknown whether bacteria can differentiate into bacteroids. The mutant creates larger nodule-like structures than those induced by early infection mutants. Bacterial penetration into epidermal cells and into the outer cortical cells surrounding the nascent nodule of mutant plants is morphologically indistinguishable from those in wild-type plants. Bacterial colonization of nodule parenchyma cells is also indistinguishable between mutants and wild type. Inf+Nod+Fix- FNB Nod+Fix- Jemalong A17 
4A-17 dnf1-2 dnf1 The mutant makes small, white nodules and has defects in nitrogen fixation. It can induce MtLEC4 and ENOD2 but not MtN31, MtCAM1, or MtLB1. The mutant forms infection threads that proceed through the cortical cells and produce limited nodule-like structures. The infection threads are indistinguishable from those of wild-type plants. It is unknown whether bacteria can differentiate into bacteroids. The mutant creates larger nodule-like structures than those induced by early infection mutants. Bacterial penetration into epidermal cells and into the outer cortical cells surrounding the nascent nodule of mutant plants is morphologically indistinguishable from those in wild-type plants. Bacterial colonization of nodule parenchyma cells is also indistinguishable between mutants and wild type. Inf+Nod+Fix- FNB Nod+Fix- Jemalong A17 

References:
Authors Year Title Locator
Mitra RM and Long SR 2004 Plant and Bacterial Symbiotic Mutants Define Three Transcriptionally Distinct Stages in the Development of the Medicago truncatula/Sinorhizobium meliloti Symbiosis Plant Physiol. 134(2):595-604 
Starker CG, Parra-Colmenares AL, Smith L, Mitra RM and Long SR 2006 Nitrogen Fixation Mutants of Medicago truncatula Fail to Support Plant and Bacterial Symbiotic Gene Expression Plant Physiol. 140(2):671-80