| Mutant Symbol |
Allele or Genotype |
Mutant Gene |
Phenotype Detail |
Symbiotic Phenotype Brief |
Mutagenesis Method |
Mutant Class |
WT Strain or Line |
|---|
| bit1-1 | bit1-1 | bit1 | defective in infection; have defects in the ability of the infection thread to penetrate past the outer cortical cells of the plant; infection threads terminated prematurely with little expansion into the nodule primordium; inoculation with Sinorhizobium meliloti resulted in the formation of small bumps on the surface of the root. bit1-1 mutants never induced any of the nodulins tested (MtLEC4, MtN31, MtCAM1, ENOD2, or MtLB1). The mutants showed no or severely diminished ENOD11:GUS induction. | Inf-Nod- | FNB | Nod- | Jemalong A17 | | bit1-2 (BC1F4) | bit1-2 (pdl1) | bit1 | root hair branching exists; develop numbers of infections comparable with wild-type plants, but fail to develop nodule primordia. The mutant displayed early arrest of infection thread growth, a phenotype reminiscent of bit1-1. width). In contrast with bit1-1, which had a wild-type root system in the absence of S. meliloti, the roots of bit1-2 had a much reduced root growth rate. Scoring for nodule structures and bacterial infection 21 d after inoculation revealed fully developed functional nodules in the wild type in contrast with bit1-2, which showed arrested infections and no noticeable inner cortical cell division. | Hab+Inf+-Ccd-Nod- | EMS | Nod- | Jemalong A17 |
|
| Authors |
Year |
Title |
Locator |
|---|
| Cohn JR, Uhm T, Ramu S, Nam YW, Kim DJ, Penmetsa RV, Wood TC, Denny RL, Young ND, Cook DR, and Stacey G | 2001 | Differential regulation of a family of apyrase genes from Medicago truncatula | Plant Physiol. 125(4):2104-19 | | Middleton PH, Jakab J, Penmetsa RV, Starker CG, Doll J, Kalo P, Prabhu R, Marsh JF, Mitra RM, Kereszt A, Dudas B, Vandenbosch K, Long SR, Cook DR, Kiss GB and Oldroyd GE | 2007 | An ERF Transcription Factor in Medicago truncatula That Is Essential for Nod Factor Signal Transduction | Plant Cell. 19(4):1221-34 | | Mitra RM and Long SR | 2004 | Plant and Bacterial Symbiotic Mutants Define Three Transcriptionally Distinct Stages in the Development of the Medicago truncatula/Sinorhizobium meliloti Symbiosis | Plant Physiol. 134(2):595-604 | | Navarro-Gochicoa MT, Camut S, Niebel A and Cullimore JV | 2003 | Expression of the apyrase-like APY1 genes in roots of Medicago truncatula is induced rapidly and transiently by stress and not by Sinorhizobium meliloti or Nod factors | Plant Physiol. 131(3):1124-36 |
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