| Mutant Symbol |
Allele or Genotype |
Mutant Gene |
Phenotype Detail |
Symbiotic Phenotype Brief |
Mutagenesis Method |
Mutant Class |
WT Strain or Line |
|---|
| C71 (domi) | dmi1-1 | dmi1 | Nod-; defective in calcium spiking; With induced touch response, the mutant showed root hair tip swelling (Has) but blocked for branching (Hab-), lack cortical cell division (Ccd-), no root curling (Hac-) and no infection threads (Inf-) in response to live bacteria; blocked in the induction of ENOD11 and ENOD40; impaired for ENOD12 expression; dramatically reduced induction of RIP1; Myc- phenotype; Recessive; Inoculation with S. meliloti was unable to elicit either superoxide production or rip1 expression; exogenous H2O2 was still able to induce rip1 expression in dmi1-1 roots. A more recent paper by Esseling et al., 2004 showed that when care is taken not to induce touch response, root hairs reorientate upon NF spot application. | Calcium flux+, Calcium spiking-, Has+, Hab-(touch response), Enod gene expression-, Ccd-, Hac-(touch response), Hac+(no touch response), Inf-, Nod-, Myc- | EMS | Nod- | Jemalong A17 | | B129 | dmi1-2 | dmi1 | Nod-; defective in calcium spiking; root hair tip swelling (Has) but are blocked for branching (Hab-); block in the induction of ENOD11 and ENOD40; dramatically reduced induction of RIP1; lack cortical cell division (Ccd-), no infection threads (Inf-), no root curling (Hac-) in response to live bacteria; Myc- phenotype; Recessive. | Calcium flux+, Calcium spiking-, Has+, Hab-, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc- | EMS | Nod- | Jemalong A17 | | Y6 | dmi1-3 | dmi1 | Nod-; defective in calcium spiking; root hair tip swelling (Has) but are blocked for branching (Hab-); block in the induction of ENOD11 and ENOD40; dramatically reduced induction of RIP1; lack cortical cell division (Ccd-), no infection threads (Inf-), no root curling (Hac-) in response to live bacteria; Myc- phenotype; Recessive | Calcium flux+, Calcium spiking-, Has+, Hab-, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc- | EMS | Nod- | Jemalong A17 | | GY15-3F-4 | dmi1-4 | dmi1 | Nod-; defective in calcium spiking; root hair tip swelling (Has) but are blocked for branching (Hab-); block in the induction of ENOD11 and ENOD40; dramatically reduced induction of RIP1; lack cortical cell division (Ccd-), no infection threads (Inf-), no root curling (Hac-) in response to live bacteria; Myc- phenotype; Recessive | Calcium flux+, Calcium spiking-, Has+, Hab-, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc- | FNB | Nod- | Jemalong A17 | | GY15-1B-5.1 | dmi1-5 | dmi1 | Nod-; defective in calcium spiking; root hair tip swelling (Has) but are blocked for branching (Hab-); block in the induction of ENOD11 and ENOD40; dramatically reduced induction of RIP1; lack cortical cell division (Ccd-), no infection threads (Inf-), no root curling (Hac-) in response to live bacteria; Myc- phenotype; Recessive | Calcium flux+, Calcium spiking-, Has+, Hab-, Enod gene expression-, Ccd-, Hac-, Inf-, Nod-, Myc- | FNB | Nod- | Jemalong A17 |
|
| Authors |
Year |
Title |
Locator |
|---|
| Ane JM, Kiss GB, Riely BK, Penmetsa RV, Oldroyd GE, Ayax C, Levy J, Debelle F, Baek JM, Kalo P, Rosenberg C, Roe BA, Long SR, Denarie J and Cook DR | 2004 | Medicago truncatula DMI1 required for bacterial and fungal symbioses in legumes | Science. 303(5662):1364-7 | | Ane JM, Levy J, Thoquet P, Kulikova O, de Billy F, Penmetsa V, Kim DJ, Debelle F, Rosenberg C, Cook DR, Bisseling T, Huguet T and Denarie J | 2002 | Genetic and cytogenetic mapping of DMI1, DMI2, and DMI3 genes of Medicago truncatula involved in Nod factor transduction, nodulation, and mycorrhization | Mol Plant Microbe Interact. 15(11):1108-18 | | Catoira R, Galera C, de Billy F, Penmetsa RV, Journet EP, Maillet F, Rosenberg C, Cook D, Gough C and Denarie J | 2000 | Four genes of Medicago truncatula controlling components of a nod factor transduction pathway | Plant Cell. 12(9):1647-66 | | Esseling JJ, Lhuissier FG and Emons AM | 2004 | A nonsymbiotic root hair tip growth phenotype in NORK-mutated legumes: implications for nodulation factor-induced signaling and formation of a multifaceted root hair pocket for bacteria | Plant Cell. 16(4):933-44 | | Mitra RM, Shaw SL and Long SR | 2004 | Six nonnodulating plant mutants defective for Nod factor-induced transcriptional changes associated with the legume-rhizobia symbiosis | Proc Natl Acad Sci U S A. 101(27):10217-22 | | Peiter E, Sun J, Heckmann AB, Venkateshwaran M, Riley BK, Otegui MS, Edwards A, Freshour G, Hahn MG, Cook DR, Sanders D, Oldroyd GE, Downie JA and Ane JM | 2007 | The Medicago truncatula DMI1 Protein Modulates Cytosolic Calcium Signaling | Plant Physiol. | | Ramu SK, Peng HM and Cook DR | 2002 | Nod factor induction of reactive oxygen species production is correlated with expression of the early nodulin gene rip1 in Medicago truncatula | Mol Plant Microbe Interact. 15(6):522-8 | | Shaw SL and Long SR | 2003 | Nod factor elicits two separable calcium responses in Medicago truncatula root hair cells | Plant Physiol. 131(3):976-84 | | Wais RJ, Galera C, Oldroyd G, Catoira R, Penmetsa RV, Cook D, Gough C, Denarie J and Long SR | 2000 | Genetic analysis of calcium spiking responses in nodulation mutants of Medicago truncatula | Proc Natl Acad Sci U S A. 97(24):13407-12 |
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